RNA

Part:BBa_K2841515:Design

Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-14)


MG1655 Specific EndA Mutant Recognition Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 158
    Illegal NgoMIV site found at 187
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The specific structure of the RNA device had to be precise otherwise the device would cease to function.


Source

DH5 Alpha EndA Mutant Information: https://microbewiki.kenyon.edu/index.php/DH5-Alpha_E.coli Genomic Information: https://www.ncbi.nlm.nih.gov/pubmed/18245285 MG1655 EndA Sequence Source: https://biocyc.org/ECOLI/sequence?type=GENE&object=EG11336

References